1 Investigation of the biphasic effect of lithium ions on HUVEC proliferation in bioactive glass extracts for burn wound regeneration 1 In the current work, we developed a simplified empirical formula to isolate and define the effect of lithium ions (Li+) released from Li-BG on the proliferation of human umbilical vein endothelial cells (HUVECs), based on experiments by Wei et al. We incorporate both culture time and concentration of Li+ (0–65.4 ppm), while providing a hormesis term to partition the characteristic biphasic (bell-shaped) cellular response to lithium. Nonlinear regression to discover best-fit parameters resulted in R2 = 0.8707, and RMSE = 0.0404. Models predict maximum stimulatory effects would occur at ~15 ppm Li⁺, which corresponds with our in vitro results that enhanced HUVEC viability occurred at 1/8–1/16 dilutions of 2.5Li-BG and 3.75Li-BG nanostructured glasses. Additionally, Monte Carlo simulation determined, for the probability of desired cellular outcomes (>80% probability HUVEC OD450 > 0.3), there exists a therapeutic window (10–15 ppm) with maximum likelihood of achieving desired cellular consequences for probabilistic models. Although the full bioactivity of Li-BG is derived from the combined expression of Li+ and Si4+ ions, as demonstrated in the original experimentation, this framework focused exclusively on Li+ allows some predictive modeling to optimize subsequent ion release profiles for next-generation wound dressings that will promote fast healing and sensory recovery. 1 4 Azadeh hasanzadeh Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz Iran hasanzadeh.azadeh@yahoo.com Mastafa H. 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