1 Probabilistic modeling of mechanical properties in thymol-loaded gelatin films for nano wound dressing applications 1 Gelatin-based films modified to contain thymol represent an exciting new bioactive material class designed for advanced wound dressing applications. Thymol provides favorable antioxidant and antimicrobial activity; however, we need a quantitative understanding of its effect on the ability of gelatin films to bear a load, which will enable the rational design of these products. The goal of this work was to develop a physics-informed probabilistic modeling framework to predict and analyze the mechanical properties (tensile strength (TS), elongation at break (EAB), and Young’s modulus (E)) of thymol-loaded gelatin films as a function of thymol concentration (0–8% w/w), using published experimental data. We fit exponential decay and saturating functions to capture the concentration dependent trends, and used a Monte Carlo simulation (5,000 trials) to quantify the uncertainty in the parameters and to estimate the probability of meeting functional mechanical thresholds (TS > 1.5 N/m², EAB > 155%, E > 2.0 MPa). Our work shows a fundamental trade-off effect: thymol provides an increase in flexibility (EAB), yet at the same time diminishes the strength and stiffness. The best range of thymol for the balance of performance is 2-4%, at which the likelihood of meeting all three criteria is highest. 1 5 Nadia Banitorfi Hoveizavi Department of Textile Engineering, Isfahan University of Technology, Isfahan, 84156-83111 Iran n.banitorfi@tx.iut.ac.ir Mastafa H. Al-Musawi Department of Biology, College of Science, Mustansiriyah University, Baghdad Iraq mustafa.h.j@uomustansiriyah.edu.iq Gelatin-based films Thymol Mechanical properties Modeling Monte Carlo simulation [1] M. Gómez-Guillén, B. Giménez, M.a. López-Caballero, M. Montero, Functional and bioactive properties of collagen and gelatin from alternative sources: A review, Food hydrocolloids 25(8) (2011) 1813-1827.##[2] J.S. Boateng, K.H. Matthews, H.N. Stevens, G.M. Eccleston, Wound healing dressings and drug delivery systems: a review, Journal of pharmaceutical sciences 97(8) (2008) 2892-2923.##[3] K. Vimala, Y.M. Mohan, K.S. Sivudu, K. Varaprasad, S. Ravindra, N.N. Reddy, Y. Padma, B. Sreedhar, K. MohanaRaju, Fabrication of porous chitosan films impregnated with silver nanoparticles: a facile approach for superior antibacterial application, Colloids and Surfaces B: Biointerfaces 76(1) (2010) 248-258.##[4] L.-H. Li, J.-C. Deng, H.-R. Deng, Z.-L. Liu, X.-L. Li, Preparation, characterization and antimicrobial activities of chitosan/Ag/ZnO blend films, Chemical Engineering Journal 160(1) (2010) 378-382.##[5] K. Can Baser, Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils, Current pharmaceutical design 14(29) (2008) 3106-3119.##[6] C.-C. Huang, H.-F. Wang, C.-H. Chen, Y.-J. Chen, K.-H. Yih, A study of four antioxidant activities and major chemical component analyses of twenty-five commonly used essential oils, Journal of Cosmetic Science 62(4) (2011) 393-404.##[7] R. García‐García, A. López‐Malo, E. Palou, Bactericidal action of binary and ternary mixtures of carvacrol, thymol, and eugenol against Listeria innocua, Journal of food science 76(2) (2011) M95-M100.##[8] J. Xu, F. Zhou, B.P. Ji, R.S. Pei, N. Xu, The antibacterial mechanism of carvacrol and thymol against Escherichia coli, Letters in applied microbiology 47(3) (2008) 174-179.##[9] A. Rao, Y. Zhang, S. Muend, R. Rao, Mechanism of antifungal activity of terpenoid phenols resembles calcium stress and inhibition of the TOR pathway, Antimicrobial agents and chemotherapy 54(12) (2010) 5062-5069.##[10] A. Ahmad, A. Khan, F. Akhtar, S. Yousuf, I. Xess, L. Khan, N. Manzoor, Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida, European journal of clinical microbiology and infectious diseases 30(1) (2011) 41-50.##[11] D. Tasdemir, M. Kaiser, F. Demirci, K. Baser, Essential oil of Turkish Origanum onites L. and its main components, carvacrol and thymol show potent antiprotozoal activity without cytotoxicity, Planta Medica 72(11) (2006) P_077.##[12] G. Kavoosi, S.M.M. Dadfar, A.M. Purfard, Mechanical, physical, antioxidant, and antimicrobial properties of gelatin films incorporated with thymol for potential use as nano wound dressing, Journal of Food Science 78(2) (2013) E244-E250.##[13] P. Tongnuanchan, S. Benjakul, T. Prodpran, Properties and antioxidant activity of fish skin gelatin film incorporated with citrus essential oils, Food chemistry 134(3) (2012) 1571-1579.